Lactose Bile Broth Granule for Coliform & E. coli Detection

Description

Lactose Bile Broth Granule: Optimized Product Description

Lactose Bile Broth Granule is a reliable and highly efficient medium designed for the detection of coliforms, fecal coliforms, and E. coli in various samples, including water, sewage, and food products. It is widely used in laboratories focused on microbial testing and quality assurance. This broth supports the growth and gas production of lactose-fermenting Gram-negative bacteria, making it an essential tool for food safety testing and environmental monitoring.

Key Ingredients and Composition

The formulation of Lactose Bile Broth Granule includes specific ingredients that promote the selective growth of coliform bacteria while inhibiting the growth of non-coliform organisms:

• Peptic digest of animal tissue (20.0 g/L): Provides essential nutrients, including nitrogen and amino acids, for bacterial growth.
• Bile salt (5.0 g/L): Inhibits non-coliform bacteria, ensuring selective growth of coliforms.
• Lactose (10.0 g/L): Acts as a fermentable carbohydrate, enabling coliform bacteria to produce gas during fermentation.
• Bromocresol purple (0.01 g/L): Serves as a pH indicator, signaling any changes in acidity due to bacterial fermentation.
• Final pH: 7.4 ± 0.2 at 25°C: The medium maintains a neutral pH, optimal for bacterial growth and fermentation processes.

This balanced formulation ensures that lactose-fermenting bacteria like E. coli can thrive, making it easier to detect their presence in water or food samples.

Preparation and Usage

Lactose Bile Broth is easy to prepare and use, making it a practical choice for both routine and high-precision microbial analysis. To prepare the broth, follow these steps:

1. Suspend 3.5 g/L of the granules in distilled or deionized water.
2. Transfer 10 mL of the prepared solution into 16mm x 150mm tubes equipped with inverted fermentation tubes.
3. Autoclave the tubes at 115°C for 15 minutes to sterilize the medium.

After cooling, the medium is ready for use in detecting coliforms and E. coli in water, sewage, and food testing applications. The broth is incubated at 36 ± 1°C for 24 ± 2 hours. Coliforms and Gram-negative bacteria that can ferment lactose will produce gas, visible as bubbles in the small inverted tubes. This visual result helps in identifying contaminants quickly and effectively.

Applications in Microbial Testing

Lactose Bile Broth Granule is extensively used in food safety, environmental testing, and water quality monitoring. It is an ideal medium for detecting fecal coliforms and E. coli in drinking water, ensuring compliance with safety regulations. In food industries, it helps monitor bacterial contamination in products, preventing the risk of foodborne illnesses. Furthermore, it serves a crucial role in testing sewage for microbial contamination, which helps maintain public health standards.

The broth’s ability to detect lactose-fermenting coliforms makes it a critical tool in laboratory quality control. Its selective components, such as bile salt and lactose, ensure that non-coliform bacteria are inhibited, allowing for more accurate results.

Quality Control and Typical Characteristics

During testing, it is important to maintain the recommended incubation conditions to ensure reliable results. Lactose Bile Broth incubated at 36 ± 1°C for 24 ± 2 hours will show gas production in the presence of lactose-fermenting coliforms like E. coli. Non-lactose fermenting bacteria, however, will not produce gas, thus providing a clear distinction between coliform and non-coliform organisms.

To ensure optimal performance, always verify the quality of the broth through regular quality control tests. Following the correct preparation and incubation methods guarantees consistent and accurate microbial detection.

Conclusion

In conclusion, Lactose Bile Broth Granule offers a precise and dependable solution for the detection of coliforms, fecal coliforms, and E. coli in food and water testing applications. Its selective ingredients, simple preparation, and efficient results make it a staple in any microbiology laboratory focused on maintaining food safety and environmental hygiene. By using Lactose Bile Broth, laboratories can ensure that their testing procedures remain accurate and aligned with industry standards.

This product stands out for its effectiveness in coliform detection, making it an essential tool for professionals in environmental monitoring and food safety.

visit our [Other Series] for more related products