Description
Kligler Iron Agar (20 Tubes) – Product Description
Kligler Iron Agar (KIA) is a vital microbiological medium. It helps identify and differentiate Enterobacteriaceae. Each package contains 20 tubes, perfect for laboratory experiments involving bacterial biochemical reactions.
Composition and Functionality
Kligler Iron Agar consists of peptone, beef extract, and yeast extract. These ingredients provide essential nitrogen sources, vitamins, and minerals for bacterial growth. The medium includes glucose and lactose, which enable acid detection through phenol red. When bacteria ferment these sugars, the medium turns yellow, indicating an acidic environment. If the medium remains red, it signifies an alkaline reaction.
Additionally, KIA detects hydrogen sulfide (H₂S) production. Some bacteria reduce sodium thiosulfate to H₂S, which reacts with iron salts. This reaction forms black ferrous sulfide, a key feature for differentiating bacterial species.
Preparation and Usage Instructions
To prepare Kligler Iron Agar, mix 55g of the powder with 1L of distilled or deionized water. Heat the mixture until it dissolves completely. Next, dispense the mixture into test tubes, filling them generously. Autoclave the tubes at 121°C for 15 minutes to sterilize. Finally, cool them to form slanted surfaces for optimal growth.
When conducting tests, inoculate the medium with fresh bacterial cultures. Use a sterile needle to streak the surface and stab the slant with the inoculum. Incubate the tubes at 36±1°C for 18-24 hours, then observe the results.
Quality Control and Microbial Sensitivity Testing
Use Kligler Iron Agar for quality control. Inoculate it with specific strains like Salmonella typhimurium, Escherichia coli, Shigella flexneri, Proteus vulgaris, and Pseudomonas aeruginosa. The expected results for these strains are as follows:
| Quality Control Strain | Strain Number | Inoculum Amount (CFU) | Reference or Counting Medium | Method | Quality Control Result | Other Characteristics |
|---|---|---|---|---|---|---|
| Salmonella typhimurium | ATCC 14028 | / | / | Qualitative | Good growth, K/A, Gas positive, H2S positive | / |
| Escherichia coli | ATCC 25922 | / | / | Qualitative | Good growth, A/A, Gas positive, H2S negative | / |
| Shigella flexneri | ATCC 12022 | / | / | Qualitative | Good growth, K/A, Gas negative, H2S negative | / |
| Proteus vulgaris | ATCC 13355 | / | / | Qualitative | Good growth, K/A, Gas positive, H2S positive | / |
| Pseudomonas aeruginosa | ATCC 27853 | / | / | Qualitative | Good growth, K/K, Gas negative, H2S negative | / |
These strains reliably assess Kligler Iron Agar’s performance in laboratory settings.
Conclusion
Kligler Iron Agar is essential for microbiologists. It helps identify and differentiate enteric bacteria based on fermentation and H₂S production. Its balanced formulation supports robust growth. The clear indicators facilitate easy interpretation of biochemical reactions. Enhance your laboratory’s capabilities with Kligler Iron Agar to streamline bacterial identification processes today! visit our [Ready-to-use culture medium] for more related products
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