Hektoen Enteric Agar (HE) for Selective Isolation of Salmonella – High-Performance Culture Medium

Additional information

Product No

Babio-4093

Specification

250g/500g

Product Use

For selective isolation (GB standard) Salmonella

Description

Hektoen Enteric Agar (HE) – Premium Culture Medium for Selective Isolation of Salmonella

Detailed Product Description

Hektoen Enteric Agar (HE) is a top-tier culture medium specifically formulated for the selective isolation of Salmonella, in compliance with GB standards. Ideal for microbiology laboratories, this agar supports food safety testing, clinical diagnostics, and research applications. Its unique formulation not only promotes the growth of Salmonella but also differentiates lactose fermenters, making it an essential tool in pathogen identification.

Key Features:

  • Selective Isolation: Specifically designed to isolate Salmonella, effectively suppressing unwanted bacteria.
  • High-Performance Ingredients:
    • Peptic Digest of Animal Tissue: 12.0 g/L for nutrient support.
    • Yeast Extract: 3.0 g/L to enhance growth.
    • Bile Salts: 9.0 g/L to inhibit non-target organisms.
    • Lactose, Sucrose, and Salicin: 12.0 g/L, 12.0 g/L, and 2.0 g/L for fermentation differentiation.
  • Color Indicators: Bromothymol blue and Acid Magenta help in visualizing growth characteristics.
  • Stable pH: Maintains a final pH of 7.5±0.2 at 25℃ for optimal microbial growth.

Preparation Instructions:

To prepare Hektoen Enteric Agar:

  1. Suspend 75.6 g of the medium in 1 L of distilled water.
  2. Boil for no more than one minute.
  3. Cool to 50℃ before pouring into sterile petri dishes.
  4. Use within 24 hours for best results. Ensure the surface of the medium remains dry.

Applications:

  • Food Microbiology: Essential for testing food samples for Salmonella contamination.
  • Clinical Microbiology: Used in hospitals and laboratories for the diagnosis of Salmonella infections.
  • Research: Valuable for studying bacterial growth patterns and pathogen behavior.
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Approximate Formula:

Ingredients gm/liter
Peptic digest of animal tissue 12.0
Yeast extract 3.0
No.3 bile 9.0
Lactose 12.0
Sucrose 12.0
Salicin 2.0
Sodium chloride 5.0
Bromothymol blue 0.065
Sodium thiosulfate 5.0
Ferric ammonium citrate 1.5
Acid Magenta 0.1
Agar 14.0
Final pH 7.5±0.2 at 25℃

*Adjusted and/or supplemented as required tomeet performance criteria.

Additional information

Product No

Babio-4093

Specification

250g/500g

Product Use

For selective isolation (GB standard) Salmonella

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