Description
Fraser Medium Granule for Listeria Enrichment Culture
Fraser Medium Granule is specifically designed for the enrichment culture of Listeria, a crucial step in microbiological analysis and food safety testing. This medium provides an ideal environment for the growth of Listeria species, enabling accurate detection and isolation. With its carefully selected components, Fraser Medium helps laboratories achieve reliable results in their testing protocols.
Key Components and Benefits
The formulation of Fraser Medium Granule includes essential nutrients that support the growth of Listeria:
- Pancreatic Casein Peptone (5.0 g/L): Provides amino acids and peptides, promoting robust microbial growth.
- Peptic Digest of Animal Tissue (5.0 g/L): Supplies additional nitrogen sources, enhancing growth conditions.
- Beef Extract Powder (5.0 g/L): Rich in vitamins and growth factors necessary for optimal Listeria development.
- Yeast Extract Powder (5.0 g/L): Contributes to the vitamin requirements of Listeria, aiding in effective enrichment.
- Sodium Chloride (20.0 g/L): Maintains osmotic balance, crucial for microbial stability.
- Disodium Hydrogen Phosphate (12.0 g/L) & Potassium Dihydrogen Phosphate (1.35 g/L): Act as buffering agents to maintain optimal pH levels.
- Esculin (1.0 g/L): Essential for specific identification of Listeria species.
- Lithium Chloride (3.0 g/L): Selectively inhibits non-target organisms, ensuring the enrichment of Listeria.
The final pH of the medium is 7.2 ± 0.2 at 25 ℃, providing an optimal environment for microbial growth.
Directions for Use
To prepare Fraser Medium Granule, suspend 55.0 g of the granules in 1 liter of distilled water. Autoclave at 121 ℃ for 15 minutes. Upon cooling to 50 ℃, dispense into 225 ml flasks. For specific applications:
- For FB1 (Half-Fraser) Medium: Add one piece of FB1 Additive A and one piece of FB1 Additive B.
- For FB2 (Fraser) Medium: Include one piece of FB2 Additive A and one piece of FB2 Additive B.
Dispense 100 ml for laboratory use, ensuring optimal conditions for Listeria enrichment.
Why Choose Fraser Medium Granule?
Choosing Fraser Medium Granule for your laboratory needs ensures that you are utilizing a product formulated for maximum efficiency in Listeria detection. The combination of high-quality ingredients and additives like FB1 and FB2 enhances both the enrichment process and the overall accuracy of your results. This medium is essential for any microbiology lab focused on food safety and pathogen detection.
Incorporating Fraser Medium Granule into your testing procedures not only improves growth yields but also streamlines the workflow for Listeria analysis, making it an indispensable tool for food safety professionals. Whether you’re working in a research facility, a quality control lab, or a diagnostic center, this medium supports your commitment to food safety and public health.
By selecting Fraser Granule, you are investing in a proven solution that meets the rigorous standards of modern microbiological testing. Enhance your Listeria enrichment culture today and ensure accurate and timely results in your laboratory processes.
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