Fluid Thioglycollate Medium Granule

Description

Fluid Thioglycollate Medium Granule for Sterility Testing and Bacterial Cultivation

Fluid Thioglycollate Medium Granule is a vital tool for the sterility testing of biologics and the cultivation of anaerobes, aerobes, and microaerophiles. Laboratories and pharmaceutical companies use it extensively due to its ability to support diverse bacterial growth. This medium ensures optimal performance by providing essential nutrients and maintaining the proper conditions for bacterial replication. It is an essential component in labs focused on sterility testing and microbial culture.

Key Ingredients and Functions

This medium contains carefully selected ingredients that meet the nutritional needs of a wide range of bacteria:

• Pancreatic Digest of Casein (15.0 g/L) provides peptides and amino acids necessary for the growth of aerobic and anaerobic organisms.
• Yeast Extract (5.0 g/L) supplies vitamins and growth factors.
• Dextrose (5.0 g/L) offers an energy source for bacteria.
• Sodium Thioglycollate (0.5 g/L) neutralizes oxygen, ensuring that anaerobic bacteria can thrive.
• L-Cystine (0.5 g/L) supports bacterial growth by helping to maintain a low redox potential.
• Sodium Chloride (2.5 g/L) maintains the osmotic balance.
• Resazurin (0.001 g/L) is a redox indicator that signals oxygen levels.
• Agar (0.75 g/L) helps stabilize the medium and maintain anaerobic conditions.

These ingredients work together to create a medium that supports the growth of both aerobic and anaerobic bacteria.

How to Prepare and Use

To prepare Fluid Thioglycollate Medium Granule, dissolve 29.25 g of the medium in one liter of distilled or deionized water. Stir and heat until fully mixed. Dispense the solution into containers, then sterilize by autoclaving at 121°C for 15 minutes. Once prepared, this medium is ideal for sterility testing and bacterial cultivation.

It is widely used in biological research, pharmaceutical quality control, and clinical diagnostics where precise conditions are crucial.

Principles of Operation

The medium supports bacterial growth through a combination of essential nutrients and a reduced redox environment. Dextrose, pancreatic digest of casein, and yeast extract supply energy and growth factors. Sodium thioglycollate and L-cystine reduce oxygen, maintaining the anaerobic environment. The redox indicator Resazurin allows visual monitoring, turning pink when oxidized and remaining colorless under anaerobic conditions.

Storage and Shelf Life

Store the granule in a tightly capped container between 5-30°C. The medium remains stable for three years from the date of manufacture. Once prepared, it can be stored at 2-8°C away from light for short-term use.

Applications in Microbiology and Sterility Testing

Fluid Thioglycollate Medium Granule is essential for sterility testing in biologics. It ensures that pharmaceuticals and vaccines meet the highest sterility standards. Additionally, it supports the cultivation of aerobic and anaerobic bacteria, making it crucial in clinical microbiology for diagnosing infections.

By integrating this medium into testing protocols, laboratories can achieve accurate, consistent results in sterility testing and bacterial culture.

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