Description
Enterobacteria Enrichment Broth Tube (10ml)
Product Overview
The Enterobacteria Enrichment Broth Tube, packaged conveniently in 10ml plastic tubes, is designed specifically for the cultivation and enrichment of enteric bacteria. This product comes in a box of 20 tubes, allowing for easy handling and transportation, ensuring a smooth workflow in microbiological laboratories.
Key Features and Benefits
Our Enterobacteria Enrichment Broth offers a nutrient-rich environment essential for the growth of specific bacteria. The formulation includes peptone, which provides vital proteins, vitamins, and amino acids necessary for microbial development. Glucose serves as the primary carbon source, promoting robust bacterial growth.
The buffer components, disodium phosphate and potassium dihydrogen phosphate, maintain optimal pH levels during incubation. Additionally, sodium deoxycholate and brilliant green act as selective inhibitors, suppressing the growth of non-Enterobacteriaceae bacteria. This selective pressure allows for the efficient isolation of target organisms.
Microbial Growth Characteristics
In the Enterobacteria Enrichment Broth, the growth of several control strains demonstrates the product’s efficacy. For instance, Escherichia coli, Enterobacter cloacae, and Salmonella typhimurium exhibit hazy, yellowish growth after incubation at 36±1℃ for 18-24 hours, confirming the broth’s suitability for enteric bacteria. In contrast, Staphylococcus aureus remains inhibited, evidenced by a lack of color change.
Control Strains | Strain Numbers | Inoculum Size (CFU) | Growth Situation | Results |
---|---|---|---|---|
Escherichia coli | ATCC 25922 | 10-100 | ++ | Hazy and yellowish |
Enterobacter cloacae | ATCC 33355 | 10-100 | ++ | Hazy and yellowish |
Salmonella typhimurium | ATCC 14028 | 10-100 | ++ | Hazy and yellowish |
Staphylococcus aureus | ATCC 25923 | 1000-5000 | – | Inhibited |
Usage Instructions
For optimal results, inoculate the Enterobacteria Enrichment Broth Tube with the desired bacterial sample and incubate under aerobic conditions at the specified temperature. After the incubation period, observe for turbidity and color change, which indicates bacterial growth and differentiation.
Conclusion
The Enterobacteria Enrichment Broth Tube is an essential tool for laboratories focusing on the isolation and identification of enteric pathogens. Its carefully formulated components not only enhance bacterial growth but also ensure selective inhibition of non-target organisms. This product is invaluable for microbiologists aiming to conduct accurate testing and analysis of enteric bacteria.
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