Enterobacter Sakazakii Chromogenic Medium for Rapid Detection

Description

nterobacter Sakazakii Chromogenic Medium

Overview

Enterobacter Sakazakii Chromogenic Medium is specifically designed for the rapid detection of Enterobacter sakazakii in infant formula and other food products. This medium allows for clear differentiation of Enterobacter sakazakii colonies, facilitating effective quality control in food safety laboratories.

Key Features

• Rapid Detection
  Enables quick identification of Enterobacter sakazakii, critical for ensuring food safety, particularly in products for infants.
• Distinct Colony Color
  Colonies of Enterobacter sakazakii appear as blue-green, while other bacteria remain colorless or yellow, simplifying the identification process.
• High Sensitivity
  The formulation supports the growth of Enterobacter sakazakii, making it a reliable choice for testing food products.

Ingredients (g/L)

• Pancreatic digest of casein: 15.0
• Soy peptone: 5.0
• Sodium chloride: 5.0
• Ferric ammonium citrate: 1.0
• Sodium thiosulfate: 1.0
• Sodium deoxycholate: 1.0
• 5-bromo-4-chloro-3-indolyl-α-D-glucopyranoside: 0.1
• Agar: 15.0

Final pH: 7.0-7.2 at 25°C

Directions for Preparation

1. Suspend 8.62 g of the medium in 200 mL of distilled water.
2. Sterilize by autoclaving at 121°C for 15 minutes.
3. Cool to approximately 50°C and pour into sterile Petri dishes.

Procedures for Use

1. Inoculate 10 μL of MSLT-Vm broth or EE enrichment broth onto the chromogenic plates using a sterile inoculation loop.
2. Invert the plates and incubate at 36 ± 1°C for 22-26 hours.
3. Examine the plates; typical Enterobacter colonies will appear as blue-green.

Quality Control

• Appearance:
  • Dehydrated powder: homogeneous, with good flowability, yellowish or milky white.
  • Prepared medium: yellowish solid.
• Microbial Sensitivity Test:
  • Incubation at 36 ± 1°C for 18-24 hours should yield blue-green colonies of sakazakii and colorless or yellow colonies for other bacteria.

Storage Recommendations

• Prepared Plates: Keep for 2-5 days; avoid direct light exposure.
• Powder Medium: Store in a dark, dry place at 2-8°C; protect from light.

Failure Criteria

Do not use dry powder media that are past their shelf life, exhibit caking, or show color variations.

visit our [Chromogenic Medium Series] for more related products