Description
Buffered Sodium Chloride Peptone Solution (pH 7.0)
Buffered Sodium Chloride Peptone Solution is an essential medium widely used in microbiology for the pre-enrichment and sample preparation of bacteria in medical applications. This solution, compliant with the European Pharmacopoeia (EP), provides optimal conditions for bacterial growth and is crucial for accurate diagnostics and research.
To prepare this solution, simply suspend 16.1 grams of the medium in one liter of distilled water. After mixing thoroughly, dispense the solution into triangle flasks, which are ideal for aeration during incubation. The next step involves sterilizing the medium in an autoclave at 121°C (15 lbs) for 15 minutes, ensuring that all contaminants are eliminated for reliable results.
Importance in Medical Microbiology
In the realm of medical microbiology, the ability to accurately culture and identify bacteria is vital. Buffered Sodium Chloride Peptone Solution effectively supports the growth of various bacterial species, allowing for precise testing and analysis. Its balanced formulation helps maintain pH stability and provides necessary nutrients, making it suitable for pre-enrichment processes.
This solution not only aids in bacterial isolation but also enhances recovery rates of stressed or injured organisms from clinical samples. By using this medium, laboratories can achieve better detection of pathogens, contributing significantly to patient diagnosis and treatment decisions.
Practical Applications
Buffered Sodium Chloride Peptone Solution is particularly beneficial in settings where bacterial detection is critical. It is commonly used in clinical laboratories, research institutions, and food safety testing. The solution enables technicians to prepare samples from different sources, including blood, urine, and environmental specimens.
By employing this buffered solution, professionals can ensure they meet regulatory standards while enhancing their testing capabilities. Moreover, its ease of preparation and sterilization makes it a convenient choice for busy laboratories.
In summary, the Buffered Sodium Chloride Peptone Solution (pH 7.0) stands out as a reliable and effective medium for bacterial sample preparation. Its application in medical microbiology facilitates the accurate identification and analysis of bacteria, supporting crucial health outcomes. For those involved in microbiological testing, incorporating this solution into your workflow can significantly enhance research and diagnostic efficacy. visit our [European Pharmacopoeia(EP)] for more related products
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