Description
Brilliant Green Lactose Bile Broth is a highly effective culture medium specifically formulated for the detection of coliform organisms in a variety of samples, including foods, dairy products, water, and wastewater. This medium plays a crucial role in ensuring food safety and maintaining water quality, making it indispensable for microbiological laboratories and testing facilities.
Key Ingredients and Composition
The formulation includes essential components designed to promote the growth of coliforms while inhibiting unwanted microbial flora:
- Peptic Digest of Animal Tissue: 10.0 g/L
- Lactose: 10.0 g/L, which facilitates lactose fermentation.
- Oxgall: 20.0 g/L, an inhibitor of gram-positive organisms.
- Brilliant Green Dye: 0.0133 g/L, another selective inhibitor for unwanted microbial growth.
The broth maintains a final pH of 7.2±0.2 at 25°C, ensuring optimal growth conditions for target organisms.
Preparation Instructions
To prepare this medium, follow these simple steps:
- Suspend 40.0 g of the dehydrated powder in 1L of purified water.
- Mix thoroughly and warm slightly to ensure complete dissolution.
- Dispense into tubes containing inverted Durham tubes to capture gas production.
- Autoclave at 121°C for 15 minutes and cool quickly to preserve medium integrity.
Principle and Interpretation
Brilliant Green Lactose Bile Broth leverages the inhibitory properties of oxgall and brilliant green dye, effectively suppressing gram-positive and certain gram-negative organisms. Only coliforms, which can ferment lactose and produce gas, thrive in this selective environment. The detection of gas production indicates successful fermentation, affirming the presence of coliforms.
Appearance
- Dehydrated Medium: A free-flowing yellowish-green powder.
- Prepared Medium: A clear green liquid, visually confirming its readiness for use.
Storage and Shelf Life
To maintain optimal performance, store Brilliant Green Lactose Bile Broth tightly capped in its original container at temperatures between 5-30°C. The dehydrated medium has a shelf life of 3 years from the date of manufacture, while the prepared medium should be stored at 2-8°C, away from direct light.
Quality Control
For effective quality assurance, inoculate and incubate the prepared medium at 36±1°C for 24-48 hours. This will confirm the growth of coliform organisms and validate the performance of the medium.
Applications
Brilliant Green Lactose Bile Broth is ideal for:
- Food Microbiology Testing: Ensuring the safety and compliance of food products.
- Water Quality Testing: Assessing drinking water and wastewater for contamination.
- Dairy Product Safety: Monitoring coliform levels in dairy products.
Reviews
There are no reviews yet.