Description
Brain Heart Infusion Broth tube (BHI) – Product Overview
Brain Heart Infusion Broth tube (BHI) is a highly nutritious culture medium that researchers and microbiologists widely use to cultivate various bacteria. With its unique formulation, BHI broth ensures robust growth and comes in convenient 10ml*20 packs. This design helps prevent contamination while allowing easy handling in laboratories and research institutions.
Key Ingredients and Composition
Each liter of BHI contains:
- Peptone: 10.0 g
- Beef Heart Infusion: 17.5 g
- Sodium Chloride: 5.0 g
- Glucose: 2.0 g
- Disodium Phosphate (12H2O): 2.5 g
The solution maintains a pH of 7.4 ± 0.2 at 25°C. This balanced composition provides essential nutrients, vitamins, and growth factors that support bacterial proliferation. Researchers appreciate that peptone and beef heart infusion serve as rich nitrogen sources, while glucose supplies energy, facilitating the growth of a wide range of bacteria.
Quality Control and Testing
To ensure product reliability, manufacturers conduct rigorous quality control testing. They utilize the GB standard microbiological sensitivity testing to confirm the medium’s effectiveness. For instance, they test various control strains, including:
| Quality Control Strain | Strain Number | Inoculum Amount (CFU) | Reference Medium | Method | Quality Control Result | Other Characteristics |
|---|---|---|---|---|---|---|
| Staphylococcus aureus | ATCC6538 | 10 – 100 | TSA | Semi-quantitative | Turbidity 2 | / |
| Escherichia coli | ATCC25922 | 10 – 100 | TSA | Semi-quantitative | Turbidity 2 | / |
| Salmonella typhimurium | ATCC14028 | 10 – 100 | TSA | Semi-quantitative | Turbidity 2 | / |
| Streptococcus pneumoniae | ATCC6305 | 10 – 100 | TSA | Semi-quantitative | Turbidity 2 | / |
| Streptococcus pyogenes | ATCC19615 | 10 – 100 | TSA | Semi-quantitative | Turbidity 2 | / |
Researchers inoculate the broth with specified control strains and incubate at 36 ± 1°C for 18-24 hours. This incubation period allows them to observe growth characteristics, confirming the efficacy of BHI in supporting bacterial cultures.
Versatile Applications
Researchers widely recognize BHI broth for its versatility in microbiological applications. It supports the growth of fastidious organisms, making it ideal for clinical, pharmaceutical, and environmental microbiology. Whether conducting bacterial culture experiments, performing sensitivity testing, or exploring pathogen identification, BHI broth serves as an essential tool in any microbiology laboratory.
Conclusion
In summary, Brain Heart Infusion Broth tube (BHI) provides a robust and reliable medium for cultivating diverse bacteria. Its carefully formulated composition and proven quality control measures ensure successful bacterial growth and experimentation. By integrating this nutrient-rich broth into your research protocols, you can achieve accurate and reproducible results in your microbiological studies. visit our [Ready-to-use culture medium] for more related products
Reviews
There are no reviews yet.