Brain Heart Infusion Broth tube BHI – Nutrient-Rich Culture Medium

Additional information

Product No

BBPT8297-51

Specification

10ml*20 pieces/box

Product Use

Nutrient-rich, for the cultivation of various bacteria

Description

Brain Heart Infusion Broth tube (BHI) – Product Overview

Brain Heart Infusion Broth tube (BHI) is a highly nutritious culture medium that researchers and microbiologists widely use to cultivate various bacteria. With its unique formulation, BHI broth ensures robust growth and comes in convenient 10ml*20 packs. This design helps prevent contamination while allowing easy handling in laboratories and research institutions.

Key Ingredients and Composition

Each liter of BHI contains:

  • Peptone: 10.0 g
  • Beef Heart Infusion: 17.5 g
  • Sodium Chloride: 5.0 g
  • Glucose: 2.0 g
  • Disodium Phosphate (12H2O): 2.5 g

The solution maintains a pH of 7.4 ± 0.2 at 25°C. This balanced composition provides essential nutrients, vitamins, and growth factors that support bacterial proliferation. Researchers appreciate that peptone and beef heart infusion serve as rich nitrogen sources, while glucose supplies energy, facilitating the growth of a wide range of bacteria.

Quality Control and Testing

To ensure product reliability, manufacturers conduct rigorous quality control testing. They utilize the GB standard microbiological sensitivity testing to confirm the medium’s effectiveness. For instance, they test various control strains, including:

Quality Control Strain Strain Number Inoculum Amount (CFU) Reference Medium Method Quality Control Result Other Characteristics
Staphylococcus aureus ATCC6538 10 – 100 TSA Semi-quantitative Turbidity 2 /
Escherichia coli ATCC25922 10 – 100 TSA Semi-quantitative Turbidity 2 /
Salmonella typhimurium ATCC14028 10 – 100 TSA Semi-quantitative Turbidity 2 /
Streptococcus pneumoniae ATCC6305 10 – 100 TSA Semi-quantitative Turbidity 2 /
Streptococcus pyogenes ATCC19615 10 – 100 TSA Semi-quantitative Turbidity 2 /

Researchers inoculate the broth with specified control strains and incubate at 36 ± 1°C for 18-24 hours. This incubation period allows them to observe growth characteristics, confirming the efficacy of BHI in supporting bacterial cultures.

Versatile Applications

Researchers widely recognize BHI broth for its versatility in microbiological applications. It supports the growth of fastidious organisms, making it ideal for clinical, pharmaceutical, and environmental microbiology. Whether conducting bacterial culture experiments, performing sensitivity testing, or exploring pathogen identification, BHI broth serves as an essential tool in any microbiology laboratory.

Conclusion

In summary, Brain Heart Infusion Broth tube (BHI) provides a robust and reliable medium for cultivating diverse bacteria. Its carefully formulated composition and proven quality control measures ensure successful bacterial growth and experimentation. By integrating this nutrient-rich broth into your research protocols, you can achieve accurate and reproducible results in your microbiological studies. visit our [Ready-to-use culture medium] for more related products

Ingredients (g/L):
Tryptone: 10.0
Beef heart infusion powder: 17.5
Sodium chloride: 5.0
Glucose: 2.0
Disodium hydrogen phosphate (12H2O): 2.5
pH value: 7.4 ± 0.2 at 25℃.

Additional information

Product No

BBPT8297-51

Specification

10ml*20 pieces/box

Product Use

Nutrient-rich, for the cultivation of various bacteria

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