Description

Brain Heart Infusion Broth (BHI) – Premium Cultivation Medium

Overview

Brain Heart Infusion Broth (BHI) is a highly nutritious infusion medium specifically designed for the cultivation of fastidious organisms, including streptococci and Neisseria. This exceptional broth supports the growth of various microorganisms, making it ideal for microbiological research and testing.

Key Ingredients and Benefits

With a carefully formulated blend of peptic digest of animal tissue, calf brain infusion, and beef heart infusion, BHI provides essential nutrients such as amino acids, vitamins, and growth factors. The inclusion of dextrose serves as an energy source, ensuring optimal growth conditions. Additionally, disodium phosphate maintains buffering capabilities, while sodium chloride preserves osmotic balance, enhancing microbial viability.

Preparation Instructions

To prepare Brain Heart Infusion Broth, simply suspend 37.0g of the medium in one liter of deionized or distilled water. After mixing well, heat with frequent agitation and boil for one minute. Finally, sterilize the solution in an autoclave at 121°C (15 lbs.) for 15 minutes to ensure a contaminant-free environment.

Storage Guidelines

For best results, store the dehydrated medium tightly capped at temperatures between 5-30°C. The shelf life extends up to three years from the manufacturing date, ensuring long-term usability. Once prepared, keep the medium out of direct light at 2-8°C.

Quality Control

Quality assurance is paramount. Upon incubation at 36±1°C for 18-24 hours, you can observe the cultural characteristics to confirm the medium’s effectiveness.

Conclusion

In summary, Brain Heart Infusion Broth (BHI) serves as an essential tool for laboratories focusing on the cultivation of fastidious organisms. Its rich nutrient profile and ease of preparation make it a preferred choice among microbiologists. Whether you’re conducting antimicrobial susceptibility tests or exploring microbial growth dynamics, BHI is your go-to medium for reliable results.

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