Baird Parker Agar Medium

Description

Optimized Product Description for Baird Parker Agar Medium

Baird Parker Agar Medium is a highly selective culture medium used for the isolation and identification of Staphylococcus aureus. It plays a crucial role in microbiological testing, particularly in food safety and clinical environments. The formulation is designed to promote the growth of Staphylococcus aureus while inhibiting the growth of unwanted organisms, making it a reliable option for laboratories.

Formula and Key Ingredients:

This medium contains a blend of ingredients that ensure selective growth:

• Pancreatic digest of casein: 10.0g/L
• Beef extract: 5.0g/L
• Yeast extract: 1.0g/L
• Sodium pyruvate: 10.0g/L
• Glycine: 12.0g/L
• Lithium chloride: 5.0g/L
• Agar: 20.0g/L
• Final pH: 6.8 ± 0.2 at 25°C

This combination encourages the development of S. aureus while suppressing other bacteria.

Preparation Instructions:

To prepare Baird Parker Agar, dissolve 58 grams of the powder in 950ml of distilled water. After mixing thoroughly, sterilize by autoclaving at 121°C for 15 minutes. Once cooled to 50°C, add 5ml of egg yolk tellurite enrichment reagent to each 95ml of agar, mixing well. Use within 48 hours of preparation to ensure reliable results.

Key Features:

When incubated at 36±1°C for 45-48 hours, S. aureus colonies form distinct features. They appear round, smooth, and convex, measuring 2-3mm in diameter. The colonies develop a dark gray to black color with a shiny surface, often surrounded by a clear, opaque zone, which is a clear indicator of S. aureus presence.

Applications and Benefits:

This medium is ideal for use in food testing laboratories, clinical settings, and environmental microbiology for accurate detection of Staphylococcus aureus. Its high specificity ensures the inhibition of unwanted bacteria, allowing for precise isolation of S. aureus. The easy preparation and dependable performance make it an essential tool in microbiological testing.

With proper storage, the dehydrated medium remains stable for three years, providing laboratories with a long-lasting and effective solution for microbial analysis. visit our [United States Pharmacopeia] for more related products