Description

Bacillus Cereus Chromogenic Medium (Generation III): Rapid Detection Solution

Bacillus Cereus Chromogenic Medium (Generation III) is the ideal solution for the rapid detection of Bacillus cereus in various products, including food, water, dairy, and meat. This medium leverages advanced chromogenic reagents and unique additives designed to enhance both specificity and sensitivity. The result? Distinctive blue-green colonies accompanied by opaque rings, making identification quick and efficient.

Key Features

The formulation consists of essential ingredients that promote optimal growth conditions for Bacillus cereus:

• Special Nutrients: 20.0 g/L
• Chromogenic Reagent: 0.5 g/L
• Bacteriostatic Agent: 4.0 g/L
• Agar: 15.0 g/L
• Final pH: 7.0 ± 0.2 at 25℃

These components work synergistically to suppress the growth of Gram-negative bacteria while allowing for the clear identification of Bacillus cereus colonies.

Enhanced Detection Process

To begin utilizing Bacillus Cereus Chromogenic Medium (Generation III), prepare your sample solution according to national standards or FDA guidelines. Enrich your sample by incubating it at 30 ± 1 ℃ for 18-24 hours. Next, transfer 25g of the sample into 225mL of GN Broth or Shigella Broth, allowing further enrichment at 36 ± 1 ℃.

After this step, streak the enriched broth onto the chromogenic medium and incubate at 36 ± 1 ℃ for another 18-24 hours. You’ll notice typical colonies of Bacillus cereus appearing blue-green with an opaque ring—an unmistakable indication of the presence of this microorganism.

Should you encounter uncertain colonies, a simple transfer to nutrient agar will allow for further biochemical testing, ensuring accurate results every time.

Quality Control and Storage

Quality assurance is crucial. The dehydrated powder should be homogeneous and flowable, while prepared media should appear as a milky-white solid. For microbial sensitivity tests, expect dark blue colonies with opaque rings for Bacillus cereus, while other strains such as Bacillus subtilis will yield white colonies without rings.

Proper storage is vital for maintaining the integrity of the medium. Prepared plates must be used immediately and kept away from direct light. Store the powdered medium in a dark, dry place at temperatures between 2-8 ℃, avoiding exposure to light to ensure long shelf life.

Limitations and Considerations

While Bacillus Cereus Chromogenic Medium (Generation III) offers rapid detection capabilities, it’s essential to note that false positives can occur. Thus, conducting supplementary tests may be necessary for confirmation. Additionally, be aware that Bacillus thuringiensis exhibits similar coloration on the medium but is rarely found in food products.

This medium serves strictly for laboratory use, making it a fundamental tool in food safety testing and microbiological analysis. Ensure your laboratory is equipped with this effective detection method to safeguard public health against Bacillus cereus contamination.

In summary, Bacillus Cereus Chromogenic Medium (Generation III) combines superior formulation with streamlined procedures, making it a top choice for laboratories seeking reliable and rapid detection of Bacillus. By integrating this medium into your workflow, you enhance the accuracy and efficiency of microbial testing, contributing to safer food and water supplies.

visit our [Chromogenic Medium Series] for more related products