Bacillus Cereus Selective Agar for Food Safety Testing

Additional information

Product No

Babio-0280

Specification

250g/500g

Product Use

Used with 50% Egg Yolk Emulsion and Polymyxin B for enumerating Bacillus cereus from foods.​

Description

Bacillus Cereus Selective Agar: Your Essential Tool for Microbial Analysis

Bacillus Cereus Selective Agar serves as a vital culture medium for effectively enumerating Bacillus cereus in food samples. This medium plays a crucial role in food safety testing, allowing for accurate detection of this bacterium, which is known to cause foodborne illnesses.

Key Features

  • Optimal Composition: The agar includes pancreatic digest of casein and beef extract, which provide essential nutrients that support the growth of Bacillus cereus while actively inhibiting unwanted microbial growth. Additionally, D-Mannitol acts as the primary carbohydrate source, enhancing the medium’s selectivity.
  • pH Indicator: With the inclusion of phenol red, the medium facilitates easy identification of mannitol fermentation. Bacillus cereus typically produces pink-red colonies, which helps users clearly differentiate it from other bacteria.
  • Enrichment with Egg Yolk: By adding 50% Egg Yolk Emulsion, you enhance lecithinase activity. This addition allows for the formation of a distinct white precipitate zone around Bacillus cereus colonies, confirming their presence with clarity.

Usage Instructions

For optimal results, first suspend 43.0 g of Bacillus Cereus Selective Agar powder in one liter of distilled water. After thorough mixing, heat the mixture, then autoclave it to ensure sterilization. Cool the medium to 40-45°C and aseptically add 100 mL of egg yolk emulsion and Polymyxin B to suppress competing flora. Finally, pour the mixture into sterile Petri dishes and allow it to solidify.

Storage and Shelf Life

To maintain integrity, store the dehydrated medium at 5-30°C in a tightly sealed container. The shelf life extends up to 3 years from the manufacturing date, while prepared media can stay fresh at 2-8°C, away from direct light.

Quality Control

For quality assurance, inoculate the medium and incubate it at 28±1°C for 24-48 hours. Observing the growth characteristics confirms the medium’s effectiveness in identifying Bacillus cereus.

Conclusion

Bacillus Cereus Selective Agar remains indispensable for laboratories focusing on food safety. Its precise formulation allows you to reliably detect Bacillus cereus, making it the preferred choice among microbiologists. Enhance your microbial analysis capabilities today with Bacillus Cereus Selective Agar, the standard in food testing environments.

Visit our [Food Hygiene Detection Series] for more related products.

Food Hygiene Detection Series Culturing Media for Safe Food Testing - Bacillus cereus, Salmonella, and E. coli Detection

Explore our Food Hygiene Detection Series, featuring specialized culturing media designed for the detection of harmful bacteria such as Bacillus cereus, Salmonella, and E. coli. Essential for food safety in restaurants and homes, our media meet the highest industry standards

Approximate Formula:

Ingredients gm/liter
Pancreatic Digest of Casein 10.0
Beef Extract 1.0
D- Mannitol 10.0
Sodium Chloride 10.0
Phenol Red 0.025
Agar 12.0
Final pH 7.2±0.2 at 25℃
*Adjusted and/or supplemented as required to meet performance criteria.

Additional information

Product No

Babio-0280

Specification

250g/500g

Product Use

Used with 50% Egg Yolk Emulsion and Polymyxin B for enumerating Bacillus cereus from foods.​

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