Description
Bacillus Cereus Selective Agar: Your Essential Tool for Microbial Analysis
Bacillus Cereus Selective Agar serves as a vital culture medium for effectively enumerating Bacillus cereus in food samples. This medium plays a crucial role in food safety testing, allowing for accurate detection of this bacterium, which is known to cause foodborne illnesses.
Key Features
- Optimal Composition: The agar includes pancreatic digest of casein and beef extract, which provide essential nutrients that support the growth of Bacillus cereus while actively inhibiting unwanted microbial growth. Additionally, D-Mannitol acts as the primary carbohydrate source, enhancing the medium’s selectivity.
- pH Indicator: With the inclusion of phenol red, the medium facilitates easy identification of mannitol fermentation. Bacillus cereus typically produces pink-red colonies, which helps users clearly differentiate it from other bacteria.
- Enrichment with Egg Yolk: By adding 50% Egg Yolk Emulsion, you enhance lecithinase activity. This addition allows for the formation of a distinct white precipitate zone around Bacillus cereus colonies, confirming their presence with clarity.
Usage Instructions
For optimal results, first suspend 43.0 g of Bacillus Cereus Selective Agar powder in one liter of distilled water. After thorough mixing, heat the mixture, then autoclave it to ensure sterilization. Cool the medium to 40-45°C and aseptically add 100 mL of egg yolk emulsion and Polymyxin B to suppress competing flora. Finally, pour the mixture into sterile Petri dishes and allow it to solidify.
Storage and Shelf Life
To maintain integrity, store the dehydrated medium at 5-30°C in a tightly sealed container. The shelf life extends up to 3 years from the manufacturing date, while prepared media can stay fresh at 2-8°C, away from direct light.
Quality Control
For quality assurance, inoculate the medium and incubate it at 28±1°C for 24-48 hours. Observing the growth characteristics confirms the medium’s effectiveness in identifying Bacillus cereus.
Conclusion
Bacillus Cereus Selective Agar remains indispensable for laboratories focusing on food safety. Its precise formulation allows you to reliably detect Bacillus cereus, making it the preferred choice among microbiologists. Enhance your microbial analysis capabilities today with Bacillus Cereus Selective Agar, the standard in food testing environments.
Visit our [Food Hygiene Detection Series] for more related products.
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