Description
Overview
Unlock the potential of yeast research with Yeast Nitrogen Base (YNB). This medium is specifically designed for classifying yeasts based on carbon assimilation. With essential nutrients included, YNB is crucial for microbiologists and biotechnologists alike.
Key Ingredients
Our Yeast Nitrogen Base contains a balanced mix of ingredients for optimal yeast growth:
- Ammonium Sulfate (5.0 g/L): A key nitrogen source that supports protein synthesis.
- Essential Amino Acids: L-Histidine, L-Methionine, and L-Tryptophan help promote robust yeast development.
- Vitamins: Includes biotin, riboflavin, and thiamine, which ensure comprehensive nutrient availability.
- Minerals: Such as boric acid, copper sulfate, and manganese sulfate support vital metabolic functions.
Preparation and Use
For best results, prepare the medium at 10× strength. Here’s how:
- Dissolve 6.7 g of YNB in 100 mL of distilled water.
- Add 5 g of dextrose or another carbohydrate source.
- Filter sterilize and store at 2-8°C.
For experimental use, dilute 0.5 mL of the 10× medium into 5 mL of sterile distilled water. Mix well to ensure even distribution.
Appearance
The dehydrated YNB appears as a free-flowing, milky-white powder. When prepared, it becomes a yellowish liquid, indicating a rich nutrient profile.
Quality Control
To maintain high quality, inoculate and incubate your prepared medium at 28±1°C for 72-120 hours. This ensures optimal yeast growth and classification.
Storage Conditions
Store the dehydrated medium tightly capped in its original container at 5-30°C. The YNB has a shelf life of 3 years from the manufacturing date, making it a reliable resource for your yeast research.
Applications
Yeast Nitrogen Base is perfect for:
- Fermentation studies
- Yeast strain classification
- Metabolic research
This medium is especially suited for laboratories focused on yeast biology, biotechnology, and brewing.
Why Choose Our Yeast Nitrogen Base?
Our YNB is crafted for researchers demanding the highest quality for yeast studies. With a formula that promotes exceptional growth and metabolic activity, it is the top choice for advancing yeast research.
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