XLD Agar for Salmonella and Shigella Isolation – Premium Microbial Growth Medium

Additional information

Product No

Babio-4105

Specification

250g/500g

Product Use

A selective medium for the isolation of salmonellae and shigellae from clinical specimens and foods

Description

XLD Agar is a highly selective culture medium designed for the isolation of Salmonella and Shigella from clinical specimens and food samples. This specialized agar supports efficient microbiological testing, ensuring accurate identification of these critical pathogens.

Key Features:

  • Selective Ingredients: XLD Agar includes sodium deoxycholate, ferric ammonium citrate, and sodium thiosulfate as selective agents to inhibit gram-positive bacteria. This ensures a pure culture environment for Salmonella and Shigella.
  • Fermentable Sugars: With xylose, lactose, and sucrose as the primary carbohydrates, XLD Agar facilitates differentiation between enteric bacteria. Salmonella ferments xylose while Shigella does not, allowing for precise identification.
  • pH Indicator: The addition of phenol red allows for visual identification of acid production, indicating successful fermentation.

Composition:

  • Yeast Extract: Provides essential nutrients and growth factors.
  • Lysine HCl: Supports the decarboxylation process, crucial for differentiation.
  • Sodium Thiosulfate: Serves as a substrate for H2S production, enabling visual confirmation of Salmonella presence.
  • Ferric Ammonium Citrate: Functions as an H2S indicator, enhancing detection accuracy.

Appearance:

  • The dehydrated medium appears as a free-flowing light red powder. Upon preparation, it forms a red, transparent gel that is visually appealing and functional.

Usage Instructions:

  1. Preparation: Suspend 53g of XLD Agar in 1L of distilled water. Heat gently while stirring until fully dissolved—avoid overheating.
  2. Cooling: Immediately transfer the medium to a water bath at 50°C.
  3. Pouring: Pour the medium into sterile Petri dishes as it cools, creating a reliable growth surface for microbial testing.

Quality Control:

Cultural characteristics must be observed after incubation at 35-37°C for 18-24 hours, ensuring reliable results. This medium is suitable for laboratories requiring stringent quality assurance in microbial testing.

Storage Conditions:

  • Store XLD Agar tightly capped in the original container at 5-30°C.
  • The dehydrated medium has a shelf life of 3 years from the manufacturing date, while prepared media should be stored at 2-8°C, away from direct light.

Why Choose XLD Agar?

XLD Agar stands out as a preferred medium for microbiologists focusing on pathogen identification in food safety and clinical diagnostics. Its unique formulation promotes selective growth, ensuring that you can accurately isolate and identify Salmonella and Shigella. With the increasing emphasis on food safety and public health, using a reliable medium like XLD Agar is essential for any microbiological laboratory.

For professionals engaged in food microbiology, clinical microbiology, and pathogen detection, XLD Agar is an indispensable tool in your lab arsenal. Optimize your microbial testing processes with our high-quality XLD Agar, ensuring the accuracy and reliability needed for public health safety.

Conclusion:

Invest in XLD Agar today and enhance your laboratory’s capacity to detect and isolate harmful pathogens effectively. As a trusted medium for Salmonella and Shigella, it not only meets performance criteria but also supports compliance with industry standards for microbial testing.

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Approximate Formula:

Ingredients gm/liter
Yeast extract 3.0
L- Lysine HCl 5.0
Lactose 7.5
Sucrose 7.5
Xylose 3.75
Sodium chloride 5.0
Sodium thiosulfate 6.8
Ferric ammonium citrate 0.8
Sodium Deoxycholate 1.0
Phenol red 0.08
Agar 12.5
Final pH 7.4±0.2 at 25℃
*Adjusted and/or supplemented as required to meet performance criteria.

Additional information

Product No

Babio-4105

Specification

250g/500g

Product Use

A selective medium for the isolation of salmonellae and shigellae from clinical specimens and foods

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