Thioglycollate Medium Tube 10ml – For Clostridium Confirmation

Description

Thioglycollate Medium Tube (FTG) for Anaerobic Bacterial Cultures – 10ml

Introduction

The Thioglycollate Medium Tube (FTG), designed for anaerobic bacterial culture, is particularly effective for confirming Clostridium perfringens and other anaerobic species. Packaged in 10ml glass tubes with secure screw caps, it minimizes contamination risks and ensures leakproof use. With a shelf life of six months, this medium is essential for laboratory environments requiring reliable anaerobic culture conditions.

Key Features and Benefits

• Comprehensive Nutrient Profile: The FTG medium includes vital nutrients such as yeast extract, casein peptone, and glucose. These ingredients support the growth of aerobic, anaerobic, and facultative anaerobic bacteria.
• Oxygen Gradient for Varied Growth Conditions: The medium creates an oxygen gradient that ranges from fully aerobic at the surface to anaerobic at the bottom, supporting a wide variety of bacterial growth.
• Redox Indicator: Resazurin, a redox indicator, provides a visual cue of the medium’s oxidation status. When oxidized, the medium turns pink, making it easy to monitor the anaerobic conditions.
• Stability with Low Agar Content: With only 0.75g of agar, the medium maintains its reduced environment while preventing oxygen diffusion, which is crucial for anaerobic bacterial growth.

Composition and Functionality

• Casein Peptone and Yeast Extract: These key components provide nitrogen and essential growth factors like amino acids and vitamins, fostering bacterial growth.
• Sodium Chloride (NaCl): Maintains osmotic balance within the medium, supporting consistent bacterial growth.
• Glucose: Offers a carbon source without significantly impacting the redox potential, ensuring stable anaerobic conditions.
• Sodium Thioglycollate: Reduces oxidative compounds and toxic peroxides, creating a conducive environment for anaerobic bacteria like Clostridium species.
• L-Cysteine: Works with sodium thioglycollate to reduce the medium’s oxidation-reduction potential, supporting the growth of strict anaerobes.
• Resazurin: Acts as a reliable redox indicator that helps identify improper storage or contamination by turning pink when exposed to oxygen.

Usage Guidelines

The Thioglycollate Medium Tube is perfect for sterility testing and anaerobic bacterial cultures. To maximize its effectiveness, follow these steps:

• Storage: Keep the tubes at room temperature to prevent degradation.
• Minimize Oxygen Exposure: Avoid shaking the tubes after sterilization to maintain the anaerobic environment.
• Check the Oxygen Layer: Before inoculation, ensure that the oxygen-exposed (pink) layer doesn’t exceed 1/5 of the tube. If necessary, heat the tube in a water bath for 20 minutes to remove the pink layer, then cool it before use.
• Fast Inoculation: Quickly inoculate the medium to prevent exposure to air and oxidation.

Practical Considerations

The FTG medium is simple to prepare and should remain clear after sterilization. If cloudiness occurs, it may indicate improper sterilization or a lower-quality agar. In such cases, review your sterilization process or change your agar supplier to maintain optimal results. visit our [Ready-to-use culture medium] for more related products