Pseudomonas CN Agar for Selective Isolation of Pseudomonas

Description

Pseudomonas CN Agar is an essential microbiological medium specifically designed for the selective isolation of Pseudomonas, with a particular emphasis on Pseudomonas aeruginosa. This agar medium is ideal for researchers and laboratories focusing on microbial studies, clinical diagnostics, and environmental monitoring, where precise identification of Pseudomonas species is crucial.

Pseudomonas CN Key Features

• Selective Isolation: Formulated to specifically isolate Pseudomonas species, enhancing recovery rates of Pseudomonas aeruginosa.
• Enhanced Pigment Production: Supports the production of characteristic pigments like pyocyanin and fluorescein, facilitating easier identification of colonies.
• Robust Nutritional Profile: Contains gelatin peptone and acid hydrolysis of casein, providing essential nutrients for optimal bacterial growth.

Composition

The approximate formula per liter includes:

• Gelatin Peptone: 16.0 g
• Acid Hydrolysis of Casein: 10.0 g
• Anhydrous Potassium Sulfate: 10.0 g
• Anhydrous Magnesium Chloride: 1.4 g
• Cetrimide: 0.2 g
• Nalidixic Acid: 0.015 g
• Agar: 13.0 g
• Final pH: 7.3±0.1 at 25℃

Directions for Use

To prepare this selective medium, suspend 50.6 g of Pseudomonas CN Agar along with 10 mL of glycerin in one liter of distilled or deionized water. Mix thoroughly and heat with frequent agitation until dissolved. Dispense into triangle flasks and sterilize using an autoclave at 121℃ (15 lbs.) for 15 minutes. The resulting prepared medium appears as a yellowish gel, ready for inoculation.

Principle and Interpretation

The unique formulation of Pseudomonas CN Agar supports the growth of Pseudomonas aeruginosa by providing essential nitrogen compounds, carbon sources, vitamins, and ions necessary for fluorescein production. The combination of magnesium and potassium ions promotes pyocyanin production, while glycerol serves as a critical energy source, enhancing overall pigment generation.

Appearance

• Dehydrated Medium: A free-flowing yellowish powder.
• Prepared Medium: A yellowish gel that provides an optimal environment for the growth of Pseudomonas species.

Precautions

This medium is strictly for laboratory use only. Avoid using dried medium that has surpassed its shelf life, is caked, or shows color variations.

Storage Conditions and Shelf Life

Store Pseudomonas CN Agar tightly capped in its original container at temperatures between 5-30°C. The dehydrated medium has a shelf life of three years from the manufacturing date. Prepared media should be stored out of direct light at 2-8°C.

Quality Control

For quality assurance, inoculate the medium and incubate at 36±1℃ for 24-48 hours. This step ensures that the medium effectively supports the growth of Pseudomonas aeruginosa and other related species, making it invaluable for microbial research and clinical applications.

Conclusion

Pseudomonas CN Agar stands out as a premier choice for laboratories engaged in the study of Pseudomonas species. Its ability to selectively isolate Pseudomonas aeruginosa while promoting pigment production makes it an essential tool for microbiologists. Whether used in clinical diagnostics or environmental assessments, this agar medium provides reliable results and supports advanced research in microbiology.

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