Nutrient Broth Granule for Bacterial Cultivation and Enumeration

Description

Nutrient Broth Granule for Bacterial Cultivation and Enumeration

Nutrient Broth Granule is a highly effective medium, commonly used for the cultivation and enumeration of non-fastidious microorganisms. It plays an essential role in laboratories testing a variety of samples, including water, food, sewage, and fecal matter. This versatile medium consistently delivers accurate results, making it indispensable for microbiological analysis in diverse industries.

Key Ingredients and Balanced Formula

The carefully designed formula of Nutrient Broth Granule provides all the nutrients bacteria need to grow. It contains:

• Peptic Digest of Animal Tissue (10.0 g/L): Supplies nitrogen compounds and amino acids, which are crucial for bacterial growth.
• Beef Extract (3.0 g/L): Provides carbon sources, vitamins, and essential trace elements that further support bacterial development.
• Sodium Chloride (5.0 g/L): Maintains the medium’s osmotic balance, ensuring cells remain stable.

The pH of 7.2 ± 0.2 at 25°C ensures an optimal growth environment for a wide variety of bacteria.

Simple Preparation and Efficient Use

Preparing Nutrient Broth Granule is straightforward. First, suspend 18.0 grams of the medium in one liter of distilled or deionized water. Then, mix it thoroughly while heating and ensure complete dissolution. After that, sterilize the medium by autoclaving at 121°C (15 lbs.) for 15 minutes. This preparation process guarantees a medium ready for microbial growth and reliable testing.

Benefits of Nutrient Broth Granule

The balanced combination of peptic digest of animal tissue and beef extract ensures that the medium provides the essential nitrogen, carbon, and vitamins necessary for bacterial growth. Additionally, sodium chloride ensures osmotic balance, which keeps bacterial cells healthy and ready to multiply. This makes Nutrient Broth Granule perfect for laboratories involved in water testing, food safety monitoring, and environmental microbiology.

Thanks to its versatility, Nutrient Broth Granule is widely used in different industries, supporting microbial analysis in both research and quality control settings. Whether you need to ensure food safety or evaluate water quality, this product consistently supports accurate bacterial enumeration.

Appearance and Quality Control

The dehydrated medium is a free-flowing yellowish granule, which turns into a clear solution once prepared. For quality assurance, inoculate the prepared broth and incubate it at 36 ± 1°C for 18-24 hours. If bacterial growth is observed, the medium is confirmed to be effective, ensuring reliability in your testing procedures.

Proper Storage and Extended Shelf Life

To maintain its effectiveness, store Nutrient Broth Granule in its original container with the lid tightly sealed. Keep the container at 5-30°C to ensure a shelf life of three years from the manufacturing date. Once the medium has been prepared, store it at 2-8°C, away from direct light, to preserve its integrity for future use.

Important Precautions

It is important to note that this medium is intended strictly for laboratory use. Be sure to check for any signs of caking or color changes before use, as these indicate that the product is no longer suitable for bacterial cultivation.

Conclusion

In summary, Nutrient Broth is a vital tool for microbiology labs focused on the cultivation and enumeration of non-fastidious bacteria. Whether you are testing water samples, assessing food safety, or conducting environmental analysis, this medium ensures reliable and repeatable results. With its balanced formula, ease of preparation, and long shelf life, Nutrient Broth Granule is a must-have for any laboratory that prioritizes consistent bacterial growth and accurate testing. visit our [Other Series] for more related products