Description

Nitrate Broth for Microbial Identification

Nitrate Broth serves as an essential tool in microbiology, specifically designed to aid the identification of aerobic and facultative anaerobic gram-negative bacteria through the nitrate reduction test. This nutrient-rich medium promotes optimal microbial growth, making it ideal for accurate testing.

Key Features of Nitrate Broth

• Enhanced Growth Environment: The formulation includes beef extract (3.0 gm/liter) and peptic digest of animal tissue (5.0 gm/liter), which provide necessary nitrogen compounds, carbon sources, and vitamins. These ingredients support the growth of various microorganisms effectively.
• Nitrate Reduction Testing: Potassium nitrate (1.0 gm/liter) enables organisms like Enterobacteriaceae to reduce nitrate to nitrite. This crucial reaction helps in identifying specific bacterial strains and determining their metabolic capabilities.
• Clear Results: When nitrate reduces to nitrite, it reacts with sulfanilic acid and N,N-dimethylα-naphthylamine, producing a distinct red color. Additionally, researchers can observe nitrogen gas formation by monitoring gas collection in Durham tubes.

Directions for Use

To prepare Nitrate Broth, suspend 9.0g of the dehydrated medium in one liter of distilled or deionized water. Mix thoroughly and heat gently for one minute. Dispense into test tubes equipped with gas-collecting Durham tubes, then sterilize at 121℃ (15 lbs.) for 15 minutes.

Storage and Shelf Life

Store Nitrate Broth tightly capped in its original container at temperatures between 5-30°C. This dehydrated medium maintains a shelf life of three years from the date of manufacture. Meanwhile, prepared medium should stay at 2-8°C, away from direct light to ensure stability.

Quality Control

For reliable results, inoculate the broth and incubate it at 36±1℃ for 24±2 hours. This quality control step ensures the effectiveness of the nitrate reduction test, which is critical for accurate microbial identification.

Approximate Formula

*Adjusted and/or supplemented as required to meet performance criteria.

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