Description
NaCl Triple Sugar Iron Tube (20 Pieces) – Essential for Biochemical Experiments
The NaCl Triple Sugar Iron Tube is a crucial tool for microbiologists focused on the selective enrichment culture of Enterobacteriaceae. Designed to facilitate biochemical testing for various bacteria, this product comes in a convenient pack of 20, ensuring you have an ample supply for your laboratory needs.
Versatile Use in Microbial Testing
This product serves as an effective medium for the biochemical examination of Vibrio, Escherichia coli, and Salmonella. The high sodium chloride concentration stabilizes the osmotic pressure, making it ideal for the growth of Vibrio species while inhibiting the growth of unwanted microorganisms.
When using the 3% NaCl Triple Sugar Iron Tube, you can easily observe the growth characteristics of Vibrio parahaemolyticus and Salmonella. Under aerobic conditions at 36±1°C for 24 hours, results show distinct patterns. For instance, Vibrio parahaemolyticus displays no gas production or H2S, while Escherichia coli produces gas without H2S. In contrast, Salmonella exhibits both gas production and H2S formation.
| Quality Control Strain | Strain Number | Inoculum Amount (CFU) | Growth Condition | Result |
|---|---|---|---|---|
| Vibrio parahaemolyticus | ATCC17802 | / | +++ | K/A no gas production, no H2S |
| Escherichia coli | ATCC25922 | / | +++ | A/A gas production, no H2S |
| Salmonella | ATCC14028 | / | +++ | K/A gas production, H2S production |
| Vibrio vulnificus | ATCC27562 | / | +++ | K/A no gas production, no H2S |
Comprehensive Testing and Results Interpretation
The NaCl Triple Sugar Iron Tube integrates several components essential for bacteria cultivation. Peptone and beef extract serve as nitrogen sources, providing essential vitamins and minerals. The fermentable sugars—lactose, glucose, and sucrose—allow for acid production, indicated by a color change in the phenol red indicator. An acidic environment turns yellow, while alkaline conditions result in a red color.
Additionally, sodium thiosulfate can be reduced by certain bacteria to produce hydrogen sulfide, which reacts with iron salts in the medium, creating a black precipitate of iron sulfide. This visual cue is vital for distinguishing between various bacterial species during biochemical testing.
Conclusion
With its unique formulation and versatile applications, the NaCl Triple Sugar Iron is an invaluable resource for microbiological studies. Whether preparing for research or routine quality control, this product ensures reliable results in the identification and differentiation of bacteria. visit our [Ready-to-use culture medium] for more related products
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