Medium 35: Microbiological Assay for Antibiotic Potency Testing

Description

Medium 35: Your Ideal Microbiological Growth Medium for Antibiotic Potency Testing

Medium 35 serves as a specialized microbiological growth medium designed for determining antibiotic potency using microbiological assay techniques. This product meets the needs of laboratories aiming for precision in antibiotic efficacy testing, ensuring reliable results that adhere to stringent research standards.

Key Ingredients and Composition

The composition of Medium 35 includes essential nutrients vital for microbial growth:

• Peptone (10.0 g/L) provides amino acids and peptides necessary for bacterial growth, promoting cell development.
• Glycerol (10.0 g/L) acts as an energy source while protecting cells against osmotic stress during experiments.
• Beef Extract (10.0 g/L) enriches the medium with vitamins and minerals that support bacterial metabolism and enhance growth.
• Sodium Chloride (3.0 g/L) maintains osmotic balance, which encourages optimal microbial growth.
• Agar (17.0 g/L) serves as a solidifying agent, facilitating easy colony isolation for accurate analysis.

This formulation achieves a final pH of 7.0 ± 0.1 at 25℃, creating an optimal environment for various microorganisms used in antibiotic testing.

Preparation Instructions

To prepare Medium 35, follow these straightforward steps:

1. Suspend 50 g of the medium in one liter of distilled water.
2. Mix thoroughly and heat with frequent agitation until complete dissolution occurs.
3. Sterilize the solution in an autoclave at 121℃ for 15 minutes to eliminate contaminants effectively.

By adhering to these preparation guidelines, you can maintain the medium’s efficacy for accurate microbiological assays.

Applications in Antibiotic Testing

Laboratories widely use Medium 35 for assessing antibiotic potency. It supports the growth of various bacteria, allowing researchers to conduct microbiological assays effectively. By relying on this medium, laboratories achieve consistent and reproducible results, which prove crucial for evaluating antibiotic effectiveness. Additionally, Medium 35 proves versatile, serving other microbiological studies beyond antibiotic potency.

Conclusion

For laboratories engaged in antibiotic testing, selecting the right growth medium is essential. Medium stands out as an effective solution for accurate microbiological assays. Its carefully balanced ingredients and straightforward preparation process provide an ideal environment for assessing antibiotic potency. Incorporating Medium 35 into your research protocols will enhance the reliability of your results and contribute to advancing antimicrobial research. visit our [United States Pharmacopeia] for more related products