Mannitol Salt Phenol Red Agar for Staphylococcus aureus Isolation

Description

Mannitol Salt Phenol Red Agar is a specialized culture medium designed for the isolation and identification of Staphylococcus aureus, particularly from foodstuffs and clinical materials. This medium is widely used in food safety testing, microbiological diagnostics, and research laboratories due to its high selectivity and differentiation capabilities.

Laboratory-grade Mannitol Salt Phenol Red Agar used for selective identification of Staphylococcus aureus in food safety testing and microbiological research

Key Features:

1. Selective for Staphylococcus aureus:
   The high concentration of sodium chloride (75g/L) inhibits the growth of non-halophilic bacteria, making this medium ideal for isolating halophilic bacteria like Staphylococcus aureus.

2. Differential Capabilities:
   The presence of D-Mannitol and Phenol Red in the medium allows for easy identification of Staphylococcus aureus. This bacterium ferments mannitol, producing acid that lowers the pH and causes the medium to change from red to yellow. This color change makes it simple to distinguish pathogenic Staphylococcus aureus colonies.

3. Comprehensive Nutrient Base:
   The inclusion of Pancreatic Digest of Casein, Peptic Digest of Animal Tissue, and Meat Extract provides essential nutrients that support bacterial growth, while maintaining a stable pH of 7.4 ± 0.2 at 25°C.

4. Versatile Applications:
   This medium is not only used in food microbiology for detecting foodborne pathogens but also in clinical diagnostics to help identify staphylococcal infections in medical specimens. It plays a critical role in ensuring the safety and quality of food products and supporting public health efforts.

How to Prepare Mannitol Salt Phenol Red Agar:

• Suspend 111g of the medium in one liter of distilled water.
• Heat the solution until boiling (approx. 1 minute), while continuously shaking to dissolve the medium completely.
• Autoclave the solution at 121°C for 15 minutes to ensure sterilization.
• Once sterilized, pour the medium into petri dishes and let it solidify.
• After cooling, the plates are ready for inoculation with bacterial samples for microbiological analysis.

Benefits of Using Mannitol Salt Phenol Red Agar:

• Accurate Identification: Ideal for detecting Staphylococcus aureus in food and clinical samples due to its selective and differential properties.
• Reliable Performance: Widely trusted in food safety labs and clinical diagnostics for staphylococcal isolation and identification.
• High Selectivity: Effectively inhibits non-target bacteria, ensuring only staphylococci can thrive.
• Proven Quality: Manufactured to meet international laboratory standards, making it suitable for research labs, hospitals, and food testing facilities.

This medium is a crucial tool in preventing foodborne illnesses caused by staphylococcal contamination, helping to maintain the safety of food supplies and enhance the efficiency of microbiological testing in professional laboratories. Whether you’re conducting food pathogen testing or clinical microbial analysis, Mannitol Salt Phenol Red Agar provides consistent and reliable results, making it an indispensable component in any lab’s toolkit.

Visit our [Food Hygiene Detection Series] for more related products.