Description
LST-MUG Tube (10ml) Product Description
Introducing the LST-MUG Tube (10ml), an advanced microbiological culture medium specifically designed for identifying and cultivating E. coli O157:H7. Packaged in a convenient 10ml plastic tube, each unit is individually wrapped to ensure leak-proof and contamination-free usage. This product is perfect for laboratories focused on food safety and microbial testing.
Product Features and Applications
The LST-MUG Tube plays a crucial role in identifying pathogenic E. coli O157:H7 strains. According to the standard SN/T0973-2000, this medium features a unique formulation that includes peptone, sodium chloride, and lactose. These ingredients provide essential carbon and nitrogen sources, promoting robust bacterial growth. Specifically, sodium chloride maintains osmotic pressure, while lactose serves as a fermentable carbohydrate.
Furthermore, potassium phosphate and disodium phosphate act as buffering agents, ensuring stability at a pH of 6.8 ± 0.2 at 25°C. A distinguishing feature of this medium is sodium lauryl sulfate, which inhibits the growth of non-E. coli bacteria, allowing for selective cultivation.
Test Principle
The test principle relies on the enzymatic activity of β-glucuronidase, found in 97% of E. coli strains and 10% of Salmonella. This enzyme hydrolyzes 4-methylumbelliferyl-β-D-glucuronide (MUG), producing blue-white fluorescence when exposed to UV light at 366nm. In contrast, E. coli O157:H7 lacks this enzyme, making it a non-fluorescent strain. This unique characteristic effectively differentiates pathogenic E. coli from other bacteria.
Medium Composition
The formulation of the LST-MUG Tube is as follows:
| Component | Concentration (g/L) |
|---|---|
| Peptone | 20.0 |
| Sodium Chloride | 5.0 |
| Lactose | 5.0 |
| Dipotassium Phosphate | 2.75 |
| Disodium Phosphate | 2.75 |
| pH Value | 6.8 ± 0.2 |
This carefully balanced formula optimizes bacterial growth while ensuring the selective detection of harmful strains.
Operation Steps and Results
For accurate results, follow these steps:
- Prepare the control culture solution.
- Introduce the control culture into the test tube.
- Incubate at 36 ± 1°C for 24 hours and record the results.
In microbiological testing, the following strains can be cultivated under similar conditions for control:
| Control Strain | Strain Number | Inoculum (CFU) | Growth Status | Other Features |
|---|---|---|---|---|
| E. coli | ATCC25922 | >5000 | +++ | Gas production, fluorescence |
| E. coli O157 | ATCC35150 | >5000 | +++ | No gas production, no fluorescence |
| Salmonella Typhi | ATCC14028 | >5000 | +++ | No gas production, no fluorescence |
| Staphylococcus aureus | ATCC25923 | >5000 | +++ | Inhibition |
Conclusion
In conclusion, the LST-MUG Tube (10ml) is an essential tool for laboratories focused on detecting and differentiating E. coli O157:H7. Its unique composition and selective growth properties make it a reliable choice for microbiological analysis. By prioritizing food safety and pathogen detection, this product meets the rigorous standards required in modern laboratory settings. visit our [Ready-to-use culture medium] for more related products
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