Description
Lactose Broth Tube (10ml)
The Lactose Broth Tube (10ml) serves as a specialized culture medium designed specifically for E. coli fermentation testing. Packaged in convenient plastic tubes, each one holds 10ml of sterile broth and comes individually wrapped. This leak-proof design prevents contamination, making the Lactose Broth Tube ideal for both laboratory and field testing.
Purpose and Applications
This Lactose Broth is primarily used to detect and enumerate Escherichia coli and other coliforms in microbiological studies. The unique formulation provides essential nutrients that support bacterial growth. Specifically, it includes peptone, which serves as a carbon and nitrogen source, fulfilling the nutritional requirements for thriving bacteria. Additionally, lactose acts as a fermentable carbohydrate, helping to identify lactose-fermenting coliforms.
Composition and Functionality
In the Lactose Broth Tube, bromocresol purple functions as a pH indicator. When bacteria ferment lactose, the medium turns yellow, indicating successful fermentation. Conversely, if the medium remains purple, it signifies alkaline conditions and no fermentation. This visual cue allows for quick and accurate interpretation of fermentation results.
Quality Control and Testing Procedure
For quality control, inoculate the broth with the following standardized strains and incubate them under aerobic conditions at 30-35°C for 24-48 hours:
Quality Control Strain | Strain Number | Inoculum (CFU) | Growth Condition | Result |
---|---|---|---|---|
Escherichia coli | CMCC(B)44102 | 10 – 100 | +++ | Acid production and gas production. |
Enterobacter aerogenes | CMCC(B)45103 | 10 – 100 | +++ | Acid production and gas production. |
Salmonella paratyphi B | CMCC(B)50094 | 10 – 100 | +++ | No acid production and no gas production. |
These results affirm the efficacy of the Lactose Broth Tube for E. coli testing and demonstrate its reliability in microbial sensitivity assays.
Key Features
- Sterile and Convenient Packaging: Each 10ml tube comes independently packaged to prevent contamination and ensure safety during transportation and use.
- High Sensitivity: This broth effectively identifies lactose fermentation, making it an excellent choice for detecting key microorganisms in various samples.
- Versatile Application: Microbiologists use this product in clinical, food, and environmental settings, emphasizing its importance for labs conducting bacteriological assessments.
In conclusion, the Lactose Broth (10ml) provides an invaluable tool for microbiologists engaged in E. coli fermentation testing and other related applications. Its user-friendly design, effective pH indicators, and reliable performance make it a top choice for laboratories striving for accurate and reproducible results. By choosing the Lactose Broth Tube, you can enhance the quality of your microbiological investigations. visit our [Ready-to-use culture medium] for more related products
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