KF Streptococcus Agar for Fecal Isolation with TTC Solution

Additional information

Product No

Babio-0268

Specification

500g

Product Use

Used with 1% TTC Solution in isolating and enumerating fecal streptococci.

Description

KF Streptococcus Agar with 1% TTC for Fecal Streptococci Isolation

Detailed Product Description

Overview: KF Streptococcus Agar is an advanced culture medium specifically formulated for the isolation and enumeration of fecal streptococci, crucial for food safety testing, water quality assessment, and clinical microbiology. Enhanced with 1% TTC (Triphenyl Tetrazolium Chloride), this medium offers superior performance in detecting fecal contamination.

Key Features:

  • High-Quality Ingredients: Formulated with proteose peptone, yeast extract, maltose, and lactose, providing a rich nutrient profile for optimal growth.
  • Selective Conditions: Sodium azide acts as a selective agent, inhibiting unwanted bacteria while promoting fecal streptococci.
  • Reliable pH: Maintains a final pH of 7.2 ± 0.2 at 25°C for optimal microbial growth.

Usage Instructions:

  1. Preparation: Dissolve 69.4g of powder in 1L of purified water. Heat with agitation until fully dissolved, then boil for 1 minute. Avoid overheating and do not autoclave.
  2. Supplementation: Cool to 50°C, then aseptically add 10 ml of 1% TTC solution. Mix thoroughly and pour into sterile plates.
  3. Incubation: Inoculate and incubate at 36 ± 1°C for 48 ± 2 hours.

Applications: KF Streptococcus Agar is essential for:

  • Microbiology Testing: Isolating fecal streptococci in food and water samples.
  • Clinical Diagnostics: Assessing fecal contamination in clinical specimens.
  • Environmental Monitoring: Ensuring water safety and quality.

Storage & Shelf Life: Store tightly capped at 5-30°C. The dehydrated medium has a shelf life of 3 years, while prepared medium should be stored at 2-8°C, protected from light.

Microorganism Strains Number Inoculum (CFU) Growth Recovery Remarks
Staphylococcus aureus ATCC 6538 100-1000 inhibited / /
Enterococcus Faecium ATCC 35667 10-100 luxuriant ≥70% Red colonies
Escherichia coli ATCC 25922 100-1000 inhibited / /
Enterococcus faecalis ATCC 29212 10-100 luxuriant ≥70% Red colonies
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Approximate Formula:

Ingredients gm/liter
Proteose Peptone 10.0
Yeast Extract 10.0
Sodium Glycerophosphate 10.0
Sodium Chloride 5.0
Maltose 20.0
Lactose 1.0
Sodium Azide 0.4
Agar 13.0
Bromocresol Purple 0.015
Final pH7.2±0.2 at 25℃
*Adjusted and/or supplemented as required to meet performance criteria.

Additional information

Product No

Babio-0268

Specification

500g

Product Use

Used with 1% TTC Solution in isolating and enumerating fecal streptococci.

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