Description
KF Streptococcus Agar with 1% TTC for Fecal Streptococci Isolation
Detailed Product Description
Overview: KF Streptococcus Agar is an advanced culture medium specifically formulated for the isolation and enumeration of fecal streptococci, crucial for food safety testing, water quality assessment, and clinical microbiology. Enhanced with 1% TTC (Triphenyl Tetrazolium Chloride), this medium offers superior performance in detecting fecal contamination.
Key Features:
- High-Quality Ingredients: Formulated with proteose peptone, yeast extract, maltose, and lactose, providing a rich nutrient profile for optimal growth.
- Selective Conditions: Sodium azide acts as a selective agent, inhibiting unwanted bacteria while promoting fecal streptococci.
- Reliable pH: Maintains a final pH of 7.2 ± 0.2 at 25°C for optimal microbial growth.
Usage Instructions:
- Preparation: Dissolve 69.4g of powder in 1L of purified water. Heat with agitation until fully dissolved, then boil for 1 minute. Avoid overheating and do not autoclave.
- Supplementation: Cool to 50°C, then aseptically add 10 ml of 1% TTC solution. Mix thoroughly and pour into sterile plates.
- Incubation: Inoculate and incubate at 36 ± 1°C for 48 ± 2 hours.
Applications: KF Streptococcus Agar is essential for:
- Microbiology Testing: Isolating fecal streptococci in food and water samples.
- Clinical Diagnostics: Assessing fecal contamination in clinical specimens.
- Environmental Monitoring: Ensuring water safety and quality.
Storage & Shelf Life: Store tightly capped at 5-30°C. The dehydrated medium has a shelf life of 3 years, while prepared medium should be stored at 2-8°C, protected from light.
Microorganism | Strains Number | Inoculum (CFU) | Growth | Recovery | Remarks |
Staphylococcus aureus | ATCC 6538 | 100-1000 | inhibited | / | / |
Enterococcus Faecium | ATCC 35667 | 10-100 | luxuriant | ≥70% | Red colonies |
Escherichia coli | ATCC 25922 | 100-1000 | inhibited | / | / |
Enterococcus faecalis | ATCC 29212 | 10-100 | luxuriant | ≥70% | Red colonies |
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