Description
Modified EC Enrichment Broth for O157 Detection
Product Specifications:
- Size: 9ml (20 tubes/box) | 10ml (20 tubes/box)
- Packaging: Plastic tubes, individually packaged for leak prevention and contamination resistance
Overview
The Modified EC Enrichment Broth is specifically designed for the selective enrichment of E. coli O157. This innovative broth plays a crucial role in food safety testing by allowing laboratories to accurately isolate and detect this pathogenic strain from food samples. Each tube contains a balanced formulation that provides the necessary nutrients for bacterial growth, ensuring reliable results.
Composition and Benefits
The formulation includes:
- Peptone: Serves as a source of carbon and nitrogen, essential for the growth of E. coli O157.
- Sodium Chloride: Maintains balanced osmotic pressure, promoting optimal growth conditions.
- Lactose: Acts as a fermentable carbohydrate, supporting the identification of lactose fermenters.
- Potassium Phosphates: These serve as buffering agents to stabilize pH levels during incubation.
- Bile Salts and Neomycin: These components inhibit the growth of common contaminants found in food, ensuring the selective enrichment of E. coli O157.
EC Enrichment Broth Testing Methodology
For microbiological sensitivity testing, inoculate the broth with the following control strains and incubate at 36±1°C in aerobic conditions for 18-24 hours:
Control Strains | Strain Numbers | Inoculum Size (CFU) | Growth Situation | Results |
---|---|---|---|---|
E. coli O157 | ATCC 35150 | 10-100 | ++ | Hazy |
Escherichia coli | ATCC 25922 | 10-100 | ++ | Hazy |
Staphylococcus aureus | ATCC 25923 | 1000-5000 | – | Inhibited |
This testing method confirms the broth’s effectiveness in promoting the growth of E. coli O157 while inhibiting non-target organisms.
Conclusion
Choosing our Modified EC Enrichment Broth ensures you have a reliable solution for the selective enrichment of E. coli O157. With individually sealed tubes, you can trust in the prevention of contamination and accurate results. Ideal for food safety laboratories, this broth helps you uphold high standards in microbiological testing. visit our [Ready-to-use culture medium] for more related products
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