Description
EC Broth Tubes with Inverted Durham Tube for Coliform and E. coli Detection
EC broth tubes with inverted Durham tubes are the ideal choice for detecting coliform bacteria and E. coli. Available in 9ml and 10ml volumes, these tubes are pre-filled and individually wrapped to prevent contamination. The design is perfect for water testing and food safety labs, ensuring accurate results without manual media preparation.
The broth supports the growth of coliforms while inhibiting Gram-positive bacteria through the use of bile salts. The inverted Durham tube makes it easy to see gas production, which indicates lactose fermentation and the presence of coliforms. This reliable product ensures consistent detection of pathogens in water and food samples.
Each tube undergoes rigorous quality control testing with reference strains like E. coli (ATCC 6538). The incubation process at 44.5°C for 24-48 hours ensures accurate identification of coliform bacteria and E. coli. Labs can trust this product for fast and reliable results in microbiological testing.
Key Benefits of EC Broth Tubes
- Ready-to-use: Sterile, pre-filled tubes save time and reduce contamination risk.
- Accurate detection: Tryptone, lactose, and bile salts ensure selectivity for coliforms while inhibiting unwanted bacteria.
- Simple gas detection: The inverted Durham tube clearly shows gas production from lactose fermentation.
- Individually wrapped: Sterile packaging prevents contamination.
- Versatile applications: Suitable for water and food safety testing, as well as general pathogen detection.
The broth contains tryptone for essential nutrients, phosphate buffers for pH stability, and sodium chloride for osmotic balance. Its formulation guarantees reliable results across a range of microbiological testing needs, including water quality and food safety.
Applications
- Water testing: Detects coliforms in drinking water, wastewater, and environmental samples.
- Food testing: Identifies E. coli contamination in food products.
- Pathogen detection: A key tool for labs testing for pathogens in various samples.
Quality Control Information for EC Broth Tubes
The EC broth tubes undergo strict quality control testing to ensure sensitivity and accuracy. The following strains are used to test the performance of the broth:
| Control Strain | Strain Number | Inoculum Size (CFU) | Growth Status | Gas Production | Result |
|---|---|---|---|---|---|
| Enterococcus faecalis | BB ATCC 6538 | 1000 | – | No gas | Inhibited |
| Escherichia coli | BB ATCC 8739 | 10–100 | +++ | Gas detected | Positive |
| Salmonella typhi | BB ATCC 14028 | 10–100 | +++ | Gas not severe | Positive |
| Proteus vulgaris | BB CMCC (B) 45103 | 10–100 | +++ | Gas not severe | Positive |
These tests ensure that the broth effectively supports the growth of coliforms while inhibiting unwanted bacteria like Enterococcus faecalis. Results from quality control indicate that Escherichia coli produces gas during fermentation, confirming positive coliform detection. Salmonella typhi and Proteus vulgaris produce minimal gas, but the presence of coliforms is still confirmed as positive.
In summary, EC broth tubes are reliable, convenient, and efficient for detecting coliforms and E. coli. Their pre-filled design, easy gas detection, and strong quality control make them essential in microbial testing and pathogen detection. Whether for water or food safety, these tubes deliver precise and consistent results. visit our [Ready-to-use culture medium] for more related products
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