Description
Brilliant Green Lactose Bile Broth (BGLB) – 10ml
Brilliant Green Lactose Bile Broth (BGLB) is a microbiological culture medium for detecting coliforms and Escherichia coli in samples. Each box contains 20 plastic tubes, each with a 10ml capacity, ensuring easy handling.
Product Overview
BGLB effectively quantifies coliforms. It is essential for food safety testing and laboratory quality control. The formulation includes peptone for carbon and nitrogen. Lactose serves as a fermentable carbohydrate. Bile salts and brilliant green dye inhibit the growth of non-enterobacteriaceae bacteria, creating a selective culture environment.
Key Features
- Packaging: User-friendly plastic tubes, 10ml each, for convenient storage.
- Test Conditions: Incubate the medium at 36±1°C under aerobic conditions for 24-48 hours. This promotes target organism growth while suppressing unwanted flora.
- Quality Control: The results are semi-quantitative, providing reliable turbidity readings and gas production for accurate microorganism identification.
Microbial Sensitivity Testing
For quality control, BGLB effectively detects several microorganisms, including:
| Control Strains | Strain Numbers | Inoculum Size (CFU) | Comparator Counting Medium | Method | Quality Control Result | Other Characteristics |
|---|---|---|---|---|---|---|
| Escherichia coli | ATCC25922 | 10-100 | TSA | Semi-quantitative | Turbidity 2, filled with gas in tube | |
| Salmonella | ATCC14028 | 10-100 | TSA | Semi-quantitative | Turbidity 2, no gas production | |
| Staphylococcus aureus | ATCC25923 | 1000-5000 | TSA | Semi-quantitative | Turbidity 0 (no growth) or turbidity 1 (weak growth), no gas production | |
| Vibrio phaseolicola | ATCC29212 | 1000-5000 | TSA | Semi-quantitative | Turbidity 0 (no growth) or turbidity 1 (weak growth), no gas production | |
| Citrobacter freundii | ATCC43864 | 10-100 | TSA | Semi-quantitative | Turbidity 2, filled with gas in 1/3 of the tube |
Applications
Laboratories use BGLB mainly for food microbiology and environmental testing. It helps ensure food products meet safety standards, thus protecting public health.
Conclusion
In summary, BGLB is essential for microbiology labs aiming to test for coliforms and E. coli accurately. Its optimized formulation and user-friendly design make it a valuable resource for food safety testing. By combining key components like lactose, bile salts, and brilliant green dye, it isolates and quantifies target bacteria efficiently. For high-quality microbiological broth, choose Brilliant Green Lactose Bile Broth for your laboratory needs. visit our [Ready-to-use culture medium] for more related products
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