Description
Brilliant Green Agar Medium for Selective Isolation of Salmonella (Excluding S. typhi and Shigella)
Brilliant Green Agar Medium is an essential tool for the isolation of Salmonella species (excluding S. typhi and Shigella). It is highly selective and widely used in clinical microbiology, food safety testing, and pharmaceutical labs. By providing a color-coded differentiation system, this medium ensures rapid identification of Salmonella. This feature makes it a trusted solution for detecting pathogens across various applications.
Key Ingredients and Formula
This medium’s formula balances nutrients and selective agents, creating an optimal environment for Salmonella growth. At the same time, it effectively inhibits other organisms. Below are the main components:
- Pancreatic Digest of Casein (5.0 g/L): Provides nitrogen and vital growth factors, crucial for bacterial development.
- Pancreatic Digest of Animal Tissue (5.0 g/L): Supplies additional nutrients that support microbial growth.
- Yeast Extract (3.0 g/L): Contributes vitamins, especially B-complex, for enhanced microbial growth.
- Sodium Chloride (5.0 g/L): Maintains osmotic balance, which is important for cell survival.
- Lactose (10.0 g/L) and Sucrose (10.0 g/L): Serve as fermentable sugars, helping identify Salmonella by fermentation reactions.
- Brilliant Green (0.0125 g/L): Inhibits Gram-positive bacteria and certain Gram-negative species, ensuring Salmonella remains unaffected.
- Phenol Red (0.08 g/L): Acts as a pH indicator, allowing quick detection of fermentation.
- Agar (20.0 g/L): Solidifies the medium, providing a stable surface for colony formation.
The medium’s final pH of 6.9 ± 0.2 at 25°C ensures an ideal growth environment for Salmonella.
Easy Preparation
To prepare Brilliant Green Agar Medium, simply suspend 58 g of the powder in 1 liter of distilled water. Then, sterilize it by autoclaving at 121°C for 15 minutes. After that, allow the medium to cool to around 50°C before pouring it into sterile Petri dishes. It’s important to use the medium on the same day you prepare it, as this ensures the best performance. For storage, always keep the medium in a dark and dry place to maintain its effectiveness.
Practical Applications
This medium excels at isolating Salmonella from a wide range of samples, including food products, water, and clinical specimens. Because of the selective properties of brilliant green, it inhibits most unwanted bacterial growth, making it easier to identify Salmonella. When incubated at 35°C for 18-24 hours, Salmonella colonies typically appear red due to the fermentation of sugars. In contrast, E. coli colonies are usually yellow to green, ensuring a clear visual distinction.
This clear color differentiation improves the accuracy of testing, making the process more efficient in laboratories focused on foodborne pathogens and clinical infections. By using this medium, you can quickly and confidently identify Salmonella, which is especially crucial in food safety and public health testing.
Quality Control and Typical Results
To ensure optimal results, perform quality control by incubating the medium at 35°C for 18-24 hours. As expected, Salmonella colonies will appear red, while E. coli and other non-target organisms will typically show up as yellow to green. These distinct color differences help quickly differentiate Salmonella from non-pathogenic bacteria, allowing for faster and more accurate identification.
Benefits of Brilliant Green Agar Medium
- Highly Selective: The medium effectively inhibits Gram-positive organisms and many Gram-negative organisms, ensuring Salmonella can grow without competition.
- Easy Identification: Thanks to the phenol red and brilliant green color indicators, you can easily distinguish Salmonella colonies.
- Versatile Applications: Whether in clinical diagnostics, food safety, or pharmaceutical testing, this medium consistently delivers reliable results. visit our [United States Pharmacopeia] for more related products
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