BPLS Agar for Selective Isolation of Salmonella | High-Performance Microbial Culture Medium

Additional information

Product No

Babio-4205

Specification

250g/500g

Product Use

Used for the selective separation and cultivation of Salmonella in pathological materials, food, feces, etc.

Description

BPLS Agar is a highly effective culture medium specifically designed for the selective isolation and cultivation of Salmonella species from various samples, including food, feces, and pathological materials. This medium is essential for laboratories focusing on food safety microbiology, pathogen detection, and clinical diagnostics.

Key Features:

  • Formulation: Each liter of BPLS Agar contains essential nutrients such as Peptic Digest of Animal Tissue, Pancreatic Digest of Casein, and Yeast Extract, which provide the necessary nitrogen and carbon sources for optimal bacterial growth.
  • Selective Agents: The inclusion of Brilliant Green acts as a selective agent that inhibits the growth of coliform bacteria and gram-positive organisms, ensuring a pure culture of Salmonella.
  • pH Indicator: Phenol Red serves as a reliable pH indicator, allowing for easy monitoring of medium conditions.
  • User-Friendly Preparation: Simply suspend 51.0g of the dehydrated medium in one liter of distilled water, sterilize, and pour into sterile petri dishes.

Application:

BPLS Agar is utilized in:

  • Food Safety Testing: Essential for detecting Salmonella in food samples, ensuring compliance with health regulations.
  • Clinical Microbiology: Vital for diagnosing infections caused by Salmonella in patients.

Storage:

Store BPLS Agar tightly capped at 5-30°C. The dehydrated medium has a shelf life of 3 years from the manufacturing date. Prepared medium should be stored at 2-8°C, away from direct light, to maintain its efficacy.

Quality Control:

To ensure performance, inoculate and incubate the medium at 36±1°C for 18-48 hours.

BPLS Agar is your go-to solution for reliable and accurate detection of Salmonella, making it a crucial addition to any laboratory’s microbiological testing repertoire.

Microorganism Strains 

Number

Inoculum

(CFU)

Growth Recovery Remarks
Salmonella enteritidis CMCC(B) 50760 10-100 luxuriant ≥70% Red coloniessurrounded

by a red zone

Salmonella typhimurium ATCC 14028 10-100 luxuriant ≥70% Red colonies surrounded

by a red zone

Escherichia coli ATCC 8739 100-1000 partial

inhibited

≤50 Yellow-green colonies

surrounded by a yellow zone

Staphylococcus aureus ATCC 6538 100-1000 inhibited / /

BPLS Agar is a medium used for the selective separation and cultivation of Salmonella in

pathological materials, food, feces, etc.

Approximate Formula:

Ingredients gm/liter
Peptic Digest of Animal Tissue 5.0
Pancreatic Digest of Casein 5.0
Yeast Extract 3.0
Sodium Chloride 5.0
Lactose 10.0
Sucrose 10.0
Phenol Red 0.08
Brilliant Green 0.0125
Agar 13.0
Final pH 6.9±0.2 at 25℃
*Adjusted and/or supplemented as required to meet performance criteria.

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Additional information

Product No

Babio-4205

Specification

250g/500g

Product Use

Used for the selective separation and cultivation of Salmonella in pathological materials, food, feces, etc.

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