Description
Blood Agar Base: Essential for Hemolytic Activity Detection
Product Overview
Blood Agar Base is a versatile and reliable medium designed for the isolation, cultivation, and detection of hemolytic activity in streptococci and other fastidious microorganisms. By adding sterile defibrinated sheep blood, this medium enhances growth factors essential for various bacteria, making it indispensable in microbiological laboratories.
Key Ingredients and Benefits
This nutrient-rich formulation includes pancreatic digest of casein and soy peptone, which supply vital nitrogen compounds, carbon sources, and trace elements necessary for bacterial growth. The inclusion of sodium chloride helps maintain osmotic balance, ensuring optimal conditions for microbial development. With a final pH of 7.3±0.1 at 25℃, Blood Agar Base creates an ideal environment for hemolysis observation.
Preparation Instructions
To prepare Blood Agar Base, simply suspend 38.0g of the medium in one liter of deionized or distilled water. Heat with frequent agitation until boiling, then sterilize in an autoclave at 121℃ (15 lbs) for 15 minutes. After cooling to 45-50℃, carefully mix in 5-10% (V/V) of sterile sheep blood before pouring into sterile petri dishes.
Applications and Interpretation
Blood Agar Base supports a wide range of applications, particularly in detecting hemolytic patterns among streptococci and other fastidious organisms. Observing different hemolytic reactions becomes straightforward, as the type of animal blood used can influence results. This makes it a critical tool in clinical diagnostics and research settings.
Storage and Shelf Life
Store Blood Agar tightly capped at temperatures between 5-30°C. The dehydrated medium has a shelf life of three years from the date of manufacture, while prepared media should be kept out of direct light at 2-8°C for optimal longevity.
Quality Control
For quality assurance, cultural characteristics should be evaluated after incubating at 36°C for 24 hours. Ensure that the medium remains free-flowing and retains its yellowish powder form prior to preparation.
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