Description
Double Lactose Bile Medium Tube (10ml)
The Double Lactose Bile Medium Tube serves as a vital tool for microbiological testing, specifically designed for detecting and enumerating coliform bacteria. This innovative medium comes packaged in convenient 10ml plastic tubes, with each tube individually sealed to prevent contamination and ensure leak-proof storage.
Key Features and Benefits
- Comprehensive Growth Support: The formulation contains peptone, which provides essential carbon and nitrogen sources to support bacterial growth. Additionally, lactose serves as a fermentable carbohydrate, crucial for identifying coliforms, particularly Escherichia coli.
- Selective Inhibition: Sodium taurocholate in the medium effectively inhibits Gram-positive bacteria. This selectivity allows you to achieve more accurate results when identifying coliforms, especially in environments with mixed bacterial populations.
- pH Indicator: Bromocresol purple acts as a pH indicator in this medium. It changes color, turning yellow in acidic conditions and purple in alkaline conditions. This visual change makes interpreting fermentation results straightforward.
Microbial Sensitivity Testing
To validate the performance of the Double Lactose Bile Medium Tube, you should conduct quality control tests with specific strains. Test these strains under aerobic conditions at 36±1°C for 24±2 hours. The following table summarizes the results for quality control strains:
| Strain | Strain Number | Inoculum (CFU) | Growth | Results |
|---|---|---|---|---|
| Escherichia coli | ATCC 25922 | 10-100 | +++ | Acid and gas production |
| Citrobacter freundii | ATCC 43864 | 10-100 | +++ | Acid production, no gas |
| Salmonella typhimurium | ATCC 14028 | 10-100 | +++ | No acid or gas production |
| Staphylococcus aureus | ATCC 6538 | 1000-5000 | – | Inhibition |
Usage Instructions
For optimal performance, inoculate the Double Lactose Bile Medium Tube with the specified quality control strains and incubate under the recommended conditions. This approach ensures accurate detection of coliforms, which is vital for food safety and public health.
Conclusion
Laboratories engaged in microbiological analysis will find the Double Lactose Bile Medium (10ml) to be an essential product. Its carefully formulated composition promotes accurate detection of coliform bacteria while supporting selective growth of target organisms. By using this medium, laboratories can confidently assess water quality, food safety, and other critical microbiological parameters. visit our [Ready-to-use culture medium] for more related products
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