Description

Enterococcus Chromogenic Medium: Rapid Detection of Enterococcus in Food, Water, and Environment

Enterococcus Chromogenic Medium is specifically designed for the rapid detection of Enterococcus species in various matrices such as food, water, and environmental samples. This innovative medium enables laboratories to efficiently identify Enterococcus, which is crucial for ensuring food safety and monitoring water quality. The unique formulation allows for the development of distinct red to purple colonies, facilitating quick visual identification.

Key Ingredients for Optimal Performance

Our formula contains essential nutrients, including:

• Nutrients: 15.0 g/L
• Tween 80: 5.0 g/L
• Chromogenic Reagent: 5.0 g/L
• Agar: 15.0 g/L

With a final pH of 7.0±0.2 at 25℃, this medium provides the ideal environment for Enterococcus growth while inhibiting unwanted microorganisms. Notably, the prepared medium showcases a yellowish solid appearance, signaling its readiness for use.

Directions for Use

To prepare the Enterococcus Chromogenic Medium, dissolve 56.4 g of the powder in 1 L of distilled water using a water bath. Once cooled to 45-50℃, pour the mixture into sterile petri dishes without the need for autoclaving. Prior to usage, allow the medium to acclimate at room temperature for 10-20 minutes.

Testing Procedures

1. Prepare a sample solution based on national standards, such as the FDA or SN guidelines.
2. Transfer 1 mL of the sample into the cooled Enterococcus Chromogenic Medium, mixing thoroughly. Alternatively, you can streak the sample directly onto the medium.
3. Incubate the plates at 36 ± 1℃ for 24-48 hours. After incubation, select 30-200 colonies and count the red to purple colonies that develop.

Quality Control and Performance

The Enterococcus Chromogenic Medium undergoes rigorous quality control to ensure reliable results. The dehydrated powder exhibits homogeneous flowability and a yellowish or milky white color, while the prepared medium appears as a solid yellowish substrate.

Microbial sensitivity tests show significant growth for Enterococcus faecalis (ATCC19433) and fecal Enterococcus (ATCC882), both presenting purple colonies. Meanwhile, contaminants like E. coli (ATCC25922) and Listeria monocytogenes (ATCC19114) do not grow on this selective medium, confirming its specificity.

Storage and Handling

Prepared plates should be used immediately and stored away from direct light. For optimal longevity, maintain the powder medium in a dark, dry place at temperatures between 2-8℃. Avoid using any dry powder media that have exceeded their shelf life, as well as those exhibiting caking or color variation.

Conclusion

Enterococcus Chromogenic Medium is an essential tool for laboratories focused on food safety and environmental monitoring. Its ability to quickly and accurately detect Enterococcus species makes it invaluable for regulatory compliance and public health initiatives. By integrating this medium into your testing protocols, you can enhance your laboratory’s efficiency and reliability in pathogen detection. Choose Enterococcus Chromogenic Medium for fast, accurate results every time.

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