Description

Kligler Agar: Essential Medium for Identifying Gram-Negative Intestinal Bacteria

Overview info:

Kligler Agar is a crucial culture medium designed for the identification of Gram-negative intestinal bacteria, particularly useful in clinical and food safety settings. Its formulation allows microbiology laboratories to accurately diagnose enteric pathogens, making it an indispensable tool in microbiological analysis.

Key Features

Kligler Agar specifically targets Gram-negative bacteria, including those common in the gastrointestinal tract. Its nutrient-rich formula contains peptones from casein and meat, yeast extract, and essential carbohydrates, which promote robust microbial growth and help differentiate between various bacterial species.

Technical Data

Composition

• Peptone from Casein: 15.0 g
• Peptone from Meat: 5.0 g
• Meat Extract: 3.0 g
• Sodium Chloride: 5.0 g
• Yeast Extract: 3.0 g
• Lactose: 10.0 g
• D(+) Glucose: 1.0 g
• Ammonium Iron(III) Citrate: 0.5 g
• Sodium Thiosulfate: 0.5 g
• Phenol Red: 0.024 g
• Agar: 12.0 g
• Final pH: 7.4 ± 0.2 at 25℃

Preparation Instructions

To prepare Kligler Agar, suspend 55 g of the medium in 1 liter of distilled water. After autoclaving at 121°C for 15 minutes, allow the medium to solidify in slants, creating an effective surface for bacterial growth.

Importance

Kligler Agar plays a pivotal role in microbiological testing by supporting the differentiation of enteric bacteria through lactose fermentation and hydrogen sulfide production. The color change indicated by phenol red helps identify lactose fermenters, providing valuable insights for diagnosing infections.

Conclusion

For laboratories focusing on the accurate identification of Gram-negative intestinal bacteria, Kligler Agar is essential. Its comprehensive nutrient profile and reliable performance ensure effective microbiological analysis, aiding in accurate diagnostics and enhancing food safety standards. Trust Kligler Agar to deliver consistent results in your microbial testing protocols.

Visit our [Food Hygiene Detection Series] for more related products.

Kligler Agar is essential for identifying Gram-negative intestinal bacteria in clinical and research settings